Typical Testing Data/Standard Curve (for reference only)
Human antigen identified by monoclonal antibody Ki-67 ELISA Kit | MKI67 elisa kit
Human Antigen KI-67, Ki-67 ELISA Kit
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Synonyms
antigen identified by monoclonal antibody Ki-67; Human Antigen KI-67; Ki-67 ELISA Kit; Human Antigen KI-67 (Ki-67) ELISA Kit; RP11-380J17.2; KIA; proliferation-related Ki-67 antigen; MKI67 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human Ki-67. No significant cross-reactivity or interference between human Ki-67 and analogues was observed.
Sequence Length
2896
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MKI67 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Ki-67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ki-67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ki-67 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ki-67 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,694 Da
NCBI Official Full Name
antigen KI-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67
UniProt Protein Name
Antigen KI-67
Protein Family
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Uniprot Description
Function: Thought to be required for maintaining cell proliferation.
Research Articles on MKI67
Similar Products
Product Notes
The Human MKI67 mki67 (Catalog #AAA706118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA706118 ELISA Kit recognizes Human MKI67. It is sometimes possible for the material contained within the vial of "antigen identified by monoclonal antibody Ki-67, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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