Mouse Macrophage inflammatory protein-3alpha ELISA Kit | MIP-3alpha elisa kit

Mouse Macrophage inflammatory protein-3alpha ELISA Kit

Cat. Num. AAA726409

• Gene Names: Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
• Reactivity: Mouse
• Synonyms: Macrophage inflammatory protein-3alpha; Mouse Macrophage inflammatory protein-3alpha ELISA Kit; Mouse Macrophage inflammatory protein-3a ELISA Kit; MIP-3alpha elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This MIP-3A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse MIP-3A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-3alpha elisa kit

Principle of the Assay: MIP-3A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MIP-3A. Standards or samples are then added to the microtiter plate wells and MIP-3A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MIP-3A present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MIP-3A are added to each well to "sandwich" the MIP-3A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MIP-3A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-3A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MIP-3alpha elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 3907618
• NCBI GeneID: 20297
• Molecular Weight: 10,755 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: Ccl20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
• NCBI Protein Information: C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: Ccl20
• UniProt Synonym Gene Names: Larc; Scya20; MIP-3-alpha
• UniProt Entry Name: CCL20_MOUSE

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; cytokine activity

Biological Process: immune response; inflammatory response; chemotaxis; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process