Typical Testing Data/Standard Curve (for reference only)
Rabbit Macrophage inflammatory protein-3alpha ELISA Kit | MIP-3alpha elisa kit
Rabbit Macrophage inflammatory protein-3alpha ELISA Kit
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Reactivity
Rabbit
Synonyms
Macrophage inflammatory protein-3alpha; Rabbit Macrophage inflammatory protein-3alpha ELISA Kit; Rabbit Macrophage inflammatory protein-3a ELISA Kit; MIP-3alpha elisa kit
Reactivity
Rabbit
Specificity
This assay has high sensitivity and excellent specificity for detection of MIP-3alpha. No significant cross-reactivity or interference between MIP-3alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MIP-3alpha and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MIP-3alpha elisa kit
Intended Uses: This MIP-3alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit MIP-3alpha. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: MIP-3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MIP-3alpha antibody and an MIP-3alpha-HRP conjugate. The assay sample and buffer are incubated together with MIP-3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP-3alpha concentration since MIP-3alpha from samples and MIP-3alpha-HRP conjugate compete for the anti-MIP-3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP-3alpha from the sample, fewer sites are left to bind MIP-3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-3alpha concentration in each sample is interpolated from this standard curve.
Principle of the Assay: MIP-3alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MIP-3alpha antibody and an MIP-3alpha-HRP conjugate. The assay sample and buffer are incubated together with MIP-3alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP-3alpha concentration since MIP-3alpha from samples and MIP-3alpha-HRP conjugate compete for the anti-MIP-3alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP-3alpha from the sample, fewer sites are left to bind MIP-3alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-3alpha concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MIP-3alpha elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,755 Da
NCBI Official Full Name
macrophage inflammatory protein 3 alpha
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 20
NCBI Official Symbol
Ccl20
NCBI Official Synonym Symbols
CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
NCBI Protein Information
C-C motif chemokine 20; MIP-3-alpha; CC chemokine LARC; CC chemokine ST38; beta chemokine exodus-1; beta-chemokine exodus-1; small-inducible cytokine A20; small inducible cytokine subfamily A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine
UniProt Protein Name
C-C motif chemokine 20
UniProt Gene Name
Ccl20
UniProt Synonym Gene Names
Larc; Scya20; MIP-3-alpha
UniProt Entry Name
CCL20_MOUSE
Uniprot Description
CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis; inflammatory response; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process
Research Articles on MIP-3alpha
Similar Products
Product Notes
The Rabbit MIP-3alpha ccl20 (Catalog #AAA721691) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA721691 ELISA Kit recognizes Rabbit MIP-3alpha. It is sometimes possible for the material contained within the vial of "Macrophage inflammatory protein-3alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
