Mindbomb Homolog 2 (MIB2) Recombinant Protein | MIB2 recombinant protein

Recombinant Mindbomb Homolog 2 (MIB2)

Cat. Num. AAA2009022

• Gene Names: Mib2; skd; Zzank1; 2210008I11Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mindbomb Homolog 2 (MIB2); Recombinant Mindbomb Homolog 2 (MIB2); MIB2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VEVLT EVPGIDVTAT NSQGFTLLHH ASLKGHVLAV RKILARARQL VDAKKEDGFT ALHLAALNNH REVAQVLIRE GRCDVNVRNR KLQSPLHLAV QQAHLGLVPL LVDAGCSVNT EDEEGDTALH VALQRHQLLP LVADRAGGDP GPLQLLSRLQ ASGLPGCTEL TVGAAVACFL ALEGADVSYA NHRGRSPLDL ATEGRVLKAL QGCAQRFRER QAGGGGGVPP GPRHVLSTPN TVTN
• Sequence Length: 921

• Applicable Applications for MIB2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val546~Asn784 (Accession # Q8R516) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 401871080
• NCBI GeneID: 76580
• NCBI Accession #: NP_001243037.2
• NCBI GenBank Nucleotide #: NM_001256108.2
• UniProt Accession #: Q8R516; Q52QU8; Q6PEF6; Q8C1N7; Q8VIB4; A2A9P9
• Molecular Weight: 29.1kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase MIB2 isoform 2
• NCBI Official Synonym Full Names: mindbomb homolog 2 (Drosophila)
• NCBI Official Symbol: Mib2
• NCBI Official Synonym Symbols: skd; Zzank1; 2210008I11Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase MIB2; dyslike; mind bomb-2; skeletrophin; dystrophin-like protein; zinc finger, ZZ type with ankyrin repeat domain 1
• UniProt Protein Name: E3 ubiquitin-protein ligase MIB2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Mib2
• UniProt Synonym Gene Names: Skd; Dyslike; Mind bomb-2
• UniProt Entry Name: MIB2_MOUSE

Uniprot Description

MIB2: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase

Cellular Component: early endosome; cytoplasm; endosome; ubiquitin ligase complex

Molecular Function: signal transducer activity; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; metal ion binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; actin binding; ligase activity

Biological Process: Notch signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein ubiquitination; signal transduction