Testing Data
Rabbit ERK1/2 (P44-MAPK) (pT202/pY204) Antibody | anti-ERK1/2 (P44-MAPK) (pT202/pY204) antibody
Rabbit anti Phospho- ERK1/2 (pThr202/pTyr204)
Reactivity
Human, Mouse, Rat, Chicken, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Synonyms
ERK1/2 (P44-MAPK) (pT202/pY204); Rabbit anti Phospho- ERK1/2 (pThr202/pTyr204); Rabbit anti--ERK1/2 (pT202/pY204) Phosphospecific; Extracellular signal-regulated kinase; ERK1; Mitogen activated protein kinase 3; MAPK3; p44/42 MAPK; ERK/MAPK; anti-ERK1/2 (P44-MAPK) (pT202/pY204) antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Canine
Specificity
This antibody recognizes ERK1/2 (pT202/pY204) with the dual phosphorylation sites. It does not cross-react with non-phosphospecific peptide.
Purity/Purification
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-ERK1/2 (P44-MAPK) (pT202/pY204) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide derived from epitope -LTEYV- with the dual phosphorylation sites Thr202 and Tyr204 of ERK1/2 protein from human, rat, mouse and dog origins.
Positive Control
UV treated 3T3 cells
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-ERK1/2 (P44-MAPK) (pT202/pY204) antibody
The extracellular signal-regulated kinases ½ (ERK1/2) are also known as MAPK (Mitogen-activated protein kinases) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (ERK1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the identification and treatment of cancer. The phosphorylation of MAPK at Thr202 and Tyr 204 are correlated to the progression and metastatic potential of tumor cells.
References
M. Klink, et al. Ovarian Cancer Cells Modulate Human Blood Neutrophils Response to Activation In Vitro. Clinical Immunology, 68 (3), 328-336. 2008.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
42/44 kDa
NCBI Official Full Name
Extracellular signal-regulated kinase 1
Similar Products
Product Notes
The ERK1/2 (P44-MAPK) (pT202/pY204) (Catalog #AAA462193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Phospho- ERK1/2 (pThr202/pTyr204) reacts with Human, Mouse, Rat, Chicken, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1/2 (P44-MAPK) (pT202/pY204) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the ERK1/2 (P44-MAPK) (pT202/pY204) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1/2 (P44-MAPK) (pT202/pY204), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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