Human major facilitator superfamily domain containing 2 ELISA Kit | MFSD2 elisa kit

Human Major facilitator superfamily domain-containing protein 2A, MFSD2 ELISA Kit

Cat. Num. AAA9324092

• Gene Names: MFSD2A; NLS1; MFSD2
• Reactivity: Human
• Synonyms: major facilitator superfamily domain containing 2; Human Major facilitator superfamily domain-containing protein 2A; MFSD2 ELISA Kit; Human Major facilitator superfamily domain-containing protein 2A (MFSD2) ELISA kit; FLJ14490; FLJ35904; MFSD2; MFSD2 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human MFSD2A and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MFSD2A concentrations in Human serum, plasma and other body fluids. Using Purified Human MFSD2A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MFSD2A and MFSD2A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MFSD2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MFSD2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MFSD2A concentrations in Human serum, plasma and other body fluids. Using Purified Human MFSD2A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MFSD2A and MFSD2A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MFSD2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 211063479
• NCBI GeneID: 84879
• NCBI Accession #: NP_001129965.1
• NCBI GenBank Nucleotide #: NM_001136493.2
• UniProt Accession #: Q8NA29; Q6UWU5; Q96F59; Q9BRC8; A8K675
• Molecular Weight: 60,170 Da
• NCBI Official Full Name: sodium-dependent lysophosphatidylcholine symporter 1 isoform 1
• NCBI Official Synonym Full Names: major facilitator superfamily domain containing 2A
• NCBI Official Symbol: MFSD2A
• NCBI Official Synonym Symbols: NLS1; MFSD2
• NCBI Protein Information: sodium-dependent lysophosphatidylcholine symporter 1; sodium-dependent LPC symporter 1; major facilitator superfamily domain-containing protein 2A
• UniProt Protein Name: Sodium-dependent lysophosphatidylcholine symporter 1
• UniProt Gene Name: MFSD2A
• UniProt Synonym Gene Names: MFSD2; NLS1; HMFN0656; PP9177; UNQ300/PRO341; NLS1; Sodium-dependent LPC symporter 1
• UniProt Entry Name: NLS1_HUMAN

Uniprot Description

MFSD2: Plays a role in thermogenesis via beta-adrenergic signaling pathway. May be the main plasma membrane tunicamycin transporter. Belongs to the major facilitator superfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: phospholipid transporter activity; symporter activity

Biological Process: phospholipid transport; transcytosis; transmembrane transport; fatty acid transport

Disease: Microcephaly 15, Primary, Autosomal Recessive