Mouse Neuregulin 4 ELISA Kit | NRG-4 elisa kit

Mouse Neuregulin 4 ELISA Kit

Cat. Num. AAA764332

• Gene Names: NRG4; HRG4
• Reactivity: Mouse
• Synonyms: Neuregulin 4; Mouse Neuregulin 4 ELISA Kit; NRG-4; NRG-4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of NRG-4 . No significant cross-reactivity or interference between NRG-4 and analogues was observed.
• Sequence Length: 115

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NRG-4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NRG-4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG-4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NRG-4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NRG-4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NRG-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NRG-4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 17068379
• NCBI GeneID: 145957
• Molecular Weight: 12,722 Da
• NCBI Official Full Name: Neuregulin 4
• NCBI Official Synonym Full Names: neuregulin 4
• NCBI Official Symbol: NRG4
• NCBI Official Synonym Symbols: HRG4
• NCBI Protein Information: pro-neuregulin-4, membrane-bound isoform
• UniProt Protein Name: Pro-neuregulin-4, membrane-bound isoform
• UniProt Gene Name: NRG4
• UniProt Synonym Gene Names: Pro-NRG4; NRG-4
• UniProt Entry Name: NRG4_HUMAN

NCBI Description

The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).[supplied by OMIM, Mar 2008]

Uniprot Description

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: extracellular region; extracellular space; integral to membrane; intracellular; plasma membrane

Molecular Function: growth factor activity; protein binding; receptor binding

Biological Process: activation of MAPKK activity; axon guidance; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; organ development; phosphoinositide-mediated signaling; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway