Human Major Facilitator Superfamily Domain Containing Protein 2A (MFSD2A) RTU ELISA Kit | MFSD2A rtu elisa kit

Human Major Facilitator Superfamily Domain Containing Protein 2A (MFSD2A) ELISA Kit

Cat. Num. AAA4500994

• Gene Names: MFSD2A; NLS1; MFSD2; MCPH15
• Reactivity: Human
• Synonyms: Major Facilitator Superfamily Domain Containing Protein 2A (MFSD2A); Human Major Facilitator Superfamily Domain Containing Protein 2A (MFSD2A) ELISA Kit; MFSD2; NLS1; Sodium-dependent lysophosphatidylcholine symporter 1; MFSD2A rtu elisa kit

• Reactivity: Human
• Sequence Length: 543

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MFSD2A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MFSD2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MFSD2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MFSD2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MFSD2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 211063479
• NCBI GeneID: 84879
• NCBI Accession #: NP_001129965.1
• NCBI GenBank Nucleotide #: NM_001136493.2
• UniProt Accession #: Q8NA29; Q6UWU5; Q96F59; Q9BRC8; A8K675
• Molecular Weight: 50,230 Da
• NCBI Official Full Name: sodium-dependent lysophosphatidylcholine symporter 1 isoform 1
• NCBI Official Synonym Full Names: major facilitator superfamily domain containing 2A
• NCBI Official Symbol: MFSD2A
• NCBI Official Synonym Symbols: NLS1; MFSD2; MCPH15
• NCBI Protein Information: sodium-dependent lysophosphatidylcholine symporter 1
• UniProt Protein Name: Sodium-dependent lysophosphatidylcholine symporter 1
• Protein Family: Sodium-dependent lysophosphatidylcholine symporter
• UniProt Gene Name: MFSD2A
• UniProt Synonym Gene Names: MFSD2; NLS1; NLS1; Sodium-dependent LPC symporter 1

NCBI Description

The protein encoded by this gene is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. The encoded protein is involved in the establishment of the blood-brain barrier and is required for brain growth and function. Defects in this gene are a cause of a progressive microcephaly syndrome. [provided by RefSeq, Mar 2017]

Uniprot Description

Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain (). Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain (PubMed:26005868). Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons (). Does not transport docosahexaenoic acid in unesterified fatty acid (). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport (). Not required for central nervous system vascular morphogenesis (). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation (PubMed:21677192). In placenta, acts as a receptor for ERVFRD-1/syncytin-2 and is required for trophoblast fusion (PubMed:18988732, PubMed:23177091).