Mouse Meteorin-like protein ELISA Kit | Metrnl elisa kit
Mouse Meteorin-like protein ELISA Kit
Reactivity
Mouse
Synonyms
Meteorin-like protein; Mouse Meteorin-like protein ELISA Kit; Metrnl/meteorin; glial cell differentiation regulator-like/meteorin-like protein/MGC99788; Metrnl elisa kit
Reactivity
Mouse
Sequence Length
311
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data
Typical Standard Curve
Related Product Information for Metrnl elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Metrnl antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Metrnl antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Metrnl amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Metrnl can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,738 Da
NCBI Official Full Name
meteorin-like protein
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator-like
NCBI Official Symbol
METRNL
NCBI Protein Information
meteorin-like protein
UniProt Protein Name
Meteorin-like protein
Protein Family
UniProt Gene Name
METRNL
UniProt Entry Name
METRL_HUMAN
Uniprot Description
METRNL: Belongs to the meteorin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: extracellular space
Molecular Function: hormone activity
Biological Process: brown fat cell differentiation; fat cell differentiation; negative regulation of inflammatory response; response to cold; response to muscle activity
