Human Meteorin-like protein ELISA Kit | METRNL elisa kit

Human Meteorin-like protein ELISA Kit

Cat. Num. AAA764456

• Reactivity: Human
• Synonyms: Meteorin-like protein; Human Meteorin-like protein ELISA Kit; METRNL/meteorin; glial cell differentiation regulator-like/meteorin-like protein/MGC99788; METRNL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of METRNL. No significant cross-reactivity or interference between METRNL and analogues was observed.
• Sequence Length: 311

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for METRNL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 52345387
• NCBI GeneID: 284207
• NCBI Accession #: NP_001004431.1
• NCBI GenBank Nucleotide #: NM_001004431.2
• Molecular Weight: 25,738 Da
• NCBI Official Full Name: meteorin-like protein
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator-like
• NCBI Official Symbol: METRNL
• NCBI Protein Information: meteorin-like protein
• UniProt Protein Name: Meteorin-like protein
• Protein Family: Meteorin-like protein
• UniProt Gene Name: METRNL
• UniProt Entry Name: METRL_HUMAN

Uniprot Description

METRNL: Belongs to the meteorin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular space

Molecular Function: hormone activity

Biological Process: brown fat cell differentiation; fat cell differentiation; negative regulation of inflammatory response; response to cold; response to muscle activity