Human Meteorin Like Protein (METRNL) ELISA Kit | METRNL elisa kit

Human Meteorin Like Protein (METRNL) ELISA Kit

Cat. Num. AAA2024853

• Reactivity: Human
• Synonyms: Meteorin Like Protein (METRNL); Human Meteorin Like Protein (METRNL) ELISA Kit; Subfatin; Glial Cell Differentiation Regulator Like; METRNL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of METRNL. No significant cross-reactivity or interference between METRNL and analogues was observed.
• Sequence Length: 311

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.63ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level METRNL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level METRNL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for METRNL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of METRNL in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to METRNL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to METRNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain METRNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of METRNL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for METRNL elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 52345387
• NCBI GeneID: 284207
• NCBI Accession #: NP_001004431.1
• NCBI GenBank Nucleotide #: NM_001004431.2
• Molecular Weight: 25,738 Da
• NCBI Official Full Name: meteorin-like protein
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator-like
• NCBI Official Symbol: METRNL
• NCBI Protein Information: meteorin-like protein
• UniProt Protein Name: Meteorin-like protein
• Protein Family: Meteorin-like protein
• UniProt Gene Name: METRNL
• UniProt Entry Name: METRL_HUMAN

Uniprot Description

METRNL: Belongs to the meteorin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular space

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to muscle activity; negative regulation of inflammatory response; brown fat cell differentiation; response to cold