SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
COVID 19 Spike RBD Coronavirus Active Protein | COVID-19 active protein
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
COVID 19 Spike RBD Coronavirus; Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; COVID-19; COVID19; HCoV-19; Human coronavirus 2019; SARS-2; SARS-CoV2; Wuhan coronavirus; SARS-CoV-2 SP RBD; 2019-nCoV SP RBD; 2019-nCoV; Spike RBD Protein; COVID-19 active protein
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
319-541aa
Sequence
Applicable Applications for COVID-19 active protein
SDS-PAGE, Bioactivity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
SARS-CoV-2 (2019-nCoV) Spike RBD
Tag
His-Tag
Predicted N-term
Ala
Bioactivity
Measured by its binding ability in a functional ELISA with Human ACE-2
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Biological Activity
(SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)
Biological Activity
(SARS-CoV-2 Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.)
Related Product Information for COVID-19 active protein
An epidemic of acute respiratory syndrome in humans, which appeared in Wuhan, China in December 2019, was caused by a novel coronavirus (SARS-CoV-2). This disease was named as "Coronavirus Disease 2019" (COVID-19). This virus shares highly homological sequence with SARS-CoV, and causes acute, highly lethal pneumonia coronavirus disease 2019 (COVID-19) with clinical symptoms similar to those reported for SARS-CoV and MERS-CoV. The genome of this and other emerging pathogenic human CoVs encodes four major structural proteins [spike (S), envelope (E), membrane (M), and nucleocapsid (N)], approximately 16 nonstructural proteins (nsp1-16), and five to eight accessory proteins. Among them, the S protein plays an essential role in viral attachment, fusion, entry, and transmission. It comprises an N-terminal S1 subunit responsible for virus-receptor binding and a C-terminal S2 subunit responsible for virus-cell membrane fusion. S1 is further divided into an N-terminal domain (NTD) and a receptor-binding domain (RBD). SARS-CoV-2 and SARS-CoV bind angiotensin-converting enzyme 2 (ACE2) while MERS-CoV binds dipeptidyl peptidase 4 (DPP4), as receptors on the host cell expressing ACE2 (e.g., pneumocytes, enterocytes) or DPP4 (e.g., liver or lung cells including Huh-7, MRC-5, and Calu-3). During infection, CoV first binds the host cell through interaction between its S1-RBD and the cell membrane receptor, triggering conformational changes in the S2 subunit that result in virus fusion and entry into the target cell. Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
References
Wu, F. et al. (2020) Nature. 579:265-269. Ortega, J.T. et al. (2020) EXCLI J. 19:410-417. Wanbo T., et al, (2020) Cell. Mol. Immunol. 17:613-620. Gorbalenya A., et al (2020) Nat. Microbiol. 5:536-544. Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93-116.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Mol_Mass: 47.9 kDa; AP_Mol_Mass: 50-60 kDa
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Product Notes
The COVID-19 (Catalog #AAA201275) is an Active Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-541aa. AAA Biotech's COVID 19 Spike RBD Coronavirus can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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