Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike S2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant MERS Spike protein (ATGP3979)
Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980)
Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981)
Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982))
Mouse MERS-CoV Spike S2 Subunit Monoclonal Antibody | anti-MERS-CoV antibody
MERS-CoV Spike S2 Subunit antibody
Reactivity
MERS-CoV
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
MERS-CoV Spike S2 Subunit; Monoclonal Antibody; MERS-CoV Spike S2 Subunit antibody; Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERS-CoV S2 P; Spike2 glycoprotein; S2 glycoprotein; S2; Spike S2 Subunit protein; S2 Subunit; anti-MERS-CoV antibody
Host
Mouse
Reactivity
MERS-CoV
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT14H8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration
1mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for anti-MERS-CoV antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
Recombinant MERS-CoV Spike S2 Subunit (752-1296aa) purified from Baculovirus
Conjugation
Unconjugated
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike S2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant MERS Spike protein (ATGP3979) Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980) Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981) Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982))
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike S2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant MERS Spike protein (ATGP3979)
Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980)
Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981)
Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982))
ELISA (EIA)
(ELISA: MERS Spike S2 Antibody (1ug/ml) specifically recognizes MERS Spike and Spike S2 recombinant protein, but not interacted MERS Spike S1 and RBD recombinant protein in ELISA.)
ELISA (EIA)
(ELISA: MERS Spike S2 Antibody (1ug/ml) specifically recognizes MERS Spike and Spike S2 recombinant protein, but not interacted MERS Spike S1 and RBD recombinant protein in ELISA.)
Related Product Information for anti-MERS-CoV antibody
MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5% based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Categories/Family for anti-MERS-CoV antibody
References
Junghyun Goo., et al. (2020) Virus Res. 278:197863. Yan-Hua Li., et al. (2019) Engineering. 5:940-947. Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017. Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202. Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.
Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. 2020 Mar;278:197863.{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114870/}
S2-14H8 mAb, which showed binding affinity to S2, showed partial neutralizing effect. (Virus Res. 2020 Mar 278:197863)
Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. Virus Res. 2020 Mar;278:197863.{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7114870/}
S2-14H8 mAb, which showed binding affinity to S2, showed partial neutralizing effect. (Virus Res. 2020 Mar 278:197863)
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The MERS-CoV (Catalog #AAA201248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MERS-CoV Spike S2 Subunit antibody reacts with MERS-CoV and may cross-react with other species as described in the data sheet. AAA Biotech's MERS-CoV Spike S2 Subunit can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the MERS-CoV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MERS-CoV Spike S2 Subunit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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