Rabbit XBP1 Polyclonal Antibody | anti-XBP1 antibody

Anti-XBP1 Antibody (aa62-91) IHC-plus

Cat. Num. AAA2400204

• Gene Names: XBP1; XBP2; TREB5; XBP-1; TREB-5
• Reactivity: Reacts: Human; Predicted reacts with: Mouse, Rat, Cow
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: XBP1; Polyclonal Antibody; Anti-XBP1 Antibody (aa62-91) IHC-plus; Rabbit Polyclonal to Human XBP1; Human XBP1; X-box binding protein 1; X-box-binding protein 1; XBP2; TREB5; XBP-1; anti-XBP1 antibody

• Host: Rabbit
• Reactivity: Reacts: Human; Predicted reacts with: Mouse, Rat, Cow
• Clonality: Polyclonal
• Specificity: XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the central region of human XBP1.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Positions: 62-91

• Applicable Applications for anti-XBP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry: (1:10 - 1:50); IHC-P: (1:100); WB: (1:1000); Optimal dilution to be determined by the researcher.
• Immunogen Species: XBP1 antibody was raised against Human.
• Antigen Modification: aa62-91
• Target Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-XBP1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(Western blot of XBP1 (arrow) using rabbit polyclonal XBP1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XBP1 gene.)

Western Blot (WB)

(Western blot of XBP1 Antibody in Ramos cell line lysates (35 ug/lane). XBP1 (arrow) was detected using the purified antibody.)

Flow Cytometry (FC/FACS)

(XBP1 Antibody flow cytometry of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-XBP1 antibody

XBP1 Antibody, X-box binding protein 1 Antibody, X-box-binding protein 1 Antibody, XBP2 Antibody, TREB5 Antibody, XBP-1 Antibody Description: XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.

NCBI and Uniprot Product Information

• NCBI GI #: 14110395
• NCBI GeneID: 7494
• NCBI Accession #: NP_005071.2
• NCBI GenBank Nucleotide #: NM_005080.3
• UniProt Accession #: P17861; Q8WYK6; Q969P1; Q96BD7
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(U)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: XBP1
• NCBI Official Synonym Symbols: XBP2; TREB5; XBP-1; TREB-5
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• Protein Family: X-box-binding protein
• UniProt Gene Name: XBP1
• UniProt Synonym Gene Names: TREB-5
• UniProt Entry Name: XBP1_HUMAN

NCBI Description

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

Uniprot Description

XBP1: a transcription factor essential for hepatocyte growth, the differentiation of plasma cells, immunoglobulin secretion, and the unfolded protein response (UPR). XBP1 mRNA is spliced by IRE1 during the UPR to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Only the spliced form of XBP1 can activate the UPR efficiently. Activates UPR target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress. Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7). Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature. Two human isoforms are produced by alternative splicing. Isoform 1 is also known as XBP-1U. Isoform 2, also known as XBP-1S, is produced by IRE1 in response to endoplasmic reticulum stress. IRE1 cleaves a 26-bp fragment causing a frameshift of the mRNA transcript.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q12.1|22q12

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to membrane; nucleoplasm; nucleus

Molecular Function: chromatin DNA binding; DNA binding; estrogen receptor binding; histone deacetylase binding; protease binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; transcription factor activity; ubiquitin protein ligase binding

Biological Process: angiogenesis; autophagy; cell growth; cellular protein metabolic process; cellular response to glucose starvation; cellular response to insulin stimulus; cellular response to nutrient; cholesterol homeostasis; endothelial cell proliferation; exocrine pancreas development; fatty acid biosynthetic process; fatty acid homeostasis; immune response; liver development; muscle development; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; neuron development; organelle organization and biogenesis; phosphoinositide 3-kinase cascade; positive regulation of autophagy; positive regulation of B cell differentiation; positive regulation of fat cell differentiation; positive regulation of histone methylation; positive regulation of immunoglobulin production; positive regulation of immunoglobulin secretion; positive regulation of MHC class II biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of T cell differentiation; positive regulation of TOR signaling pathway; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein destabilization; protein transport; regulation of autophagy; regulation of protein stability; sterol homeostasis; transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; unfolded protein response; vascular endothelial growth factor receptor signaling pathway

Disease: Major Affective Disorder 7