Human matrix extracellular phosphoglycoprotein ELISA Kit | MEPE elisa kit

Human matrix extracellular phosphoglycoprotein, MEPE ELISA Kit

Cat. Num. AAA703910

• Gene Names: MEPE; OF45
• Reactivity: Human
• Synonyms: matrix extracellular phosphoglycoprotein; Human matrix extracellular phosphoglycoprotein; MEPE ELISA Kit; OF45; matrix; extracellular phosphoglycoprotein with ASARM motif (bone) ; MEPE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MEPE. No significant cross-reactivity or interference between Human MEPE and analogues was observed.
• Sequence Length: 525

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 15.6 ng/ml-1000 ng/ml
• Sensitivity: 3.9 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MEPE elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MEPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MEPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEPE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEPE bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

Radiation activated CHK1/MEPE pathway may contribute to microgravity-induced bone density loss. Xiangming Zhang, Ping Wang, Ya Wang (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 296011076
• NCBI GeneID: 56955
• NCBI Accession #: NP_001171623.1
• NCBI GenBank Nucleotide #: NM_001184694.2
• UniProt Accession #: Q9NQ76; A1A4X9; A8MTA3; D2CFR4; F5H5C5
• Molecular Weight: 58,419 Da
• NCBI Official Full Name: matrix extracellular phosphoglycoprotein isoform a
• NCBI Official Synonym Full Names: matrix extracellular phosphoglycoprotein
• NCBI Official Symbol: MEPE
• NCBI Official Synonym Symbols: OF45
• NCBI Protein Information: matrix extracellular phosphoglycoprotein; osteoblast/osteocyte factor 45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone)
• UniProt Protein Name: Matrix extracellular phosphoglycoprotein
• Protein Family: Matrix extracellular phosphoglycoprotein
• UniProt Gene Name: MEPE
• UniProt Synonym Gene Names: OF45
• UniProt Entry Name: MEPE_HUMAN

NCBI Description

This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

Function: Promotes renal phosphate excretion and modulates mineralization.1 PublicationManual assertion based on experiment in:Ref.7