SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SPARC-like 1/SPARCL1 Recombinant Protein | SPARCL1 recombinant protein
Recombinant SPARC-like 1/SPARCL1
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
SPARC-like 1/SPARCL1; Recombinant SPARC-like 1/SPARCL1; Sparcl1; Ecm2; hevin; mast9; Sc1; SPARC-like protein 1; Extracellular matrix protein 2; Matrix glycoprotein Sc1; SPARCL1 recombinant protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
17-650aa
Sequence
IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLF LEHHHH HH
Sequence Length
650
Applicable Applications for SPARCL1 recombinant protein
SDS-PAGE
Species
Mouse
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ile 17
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for SPARCL1 recombinant protein
SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Recombinant Mouse SPARCL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for SPARCL1 recombinant protein
References
Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991-996. Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735-748.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71.7kDa (642aa)
NCBI Official Full Name
SPARC-like protein 1
UniProt Protein Name
SPARC-like protein 1
Protein Family
UniProt Gene Name
Sparcl1
UniProt Synonym Gene Names
Ecm2; Sc1
UniProt Entry Name
SPRL1_MOUSE
Uniprot Description
SPARCL1: Belongs to the SPARC family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix; synapse
Molecular Function: calcium ion binding; metal ion binding
Biological Process: signal transduction
Similar Products
Product Notes
The SPARCL1 sparcl1 (Catalog #AAA201156) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 17-650aa. AAA Biotech's SPARC-like 1/SPARCL1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SPARCL1 sparcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLF LEHHHH HH. It is sometimes possible for the material contained within the vial of "SPARC-like 1/SPARCL1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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