Rabbit anti-Human VAMP7 Polyclonal Antibody | anti-VAMP7 antibody

VAMP7, ID (VAMP7, SYBL1, Vesicle-associated membrane protein 7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP) (Azide free) (HRP)

Cat. Num. AAA6362217

• Gene Names: VAMP7; SYBL1; TIVAMP; VAMP-7; TI-VAMP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VAMP7; Polyclonal Antibody; ID (VAMP7; SYBL1; Vesicle-associated membrane protein 7; Synaptobrevin-like protein 1; Tetanus-insensitive VAMP) (Azide free) (HRP); Tetanus-insensitive VAMP; anti-VAMP7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VAMP7 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: VAMP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the Central region of human VAMP7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VAMP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the VAMP7 antibody detected the VAMP7 protein (arrow).)

Immunohistochemistry (IHC)

(VAMP7 Antibody (Center) (AP13865c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VAMP7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-VAMP7 antibody

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-VAMP7 antibody

Antibodies; Membrane Proteins

References

Vivona, S., et al. J. Biol. Chem. 285(23):17965-17973(2010). Danglot, L., et al. J. Cell. Sci. 123 (PT 5), 723-735 (2010): Fader, C.M., et al. Biochim. Biophys. Acta 1793(12):1901-1916(2009). Burgo, A., et al. EMBO Rep. 10(10):1117-1124(2009). Ward, D.M., et al. Mol. Biol. Cell 11(7):2327-2333(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 223468574
• NCBI GeneID: 6845
• NCBI Accession #: NP_001138621.1; NP_005629.1; NP_001172112.1
• NCBI GenBank Nucleotide #: NM_001145149.2
• UniProt Accession #: P51809; Q53GY7; Q7Z409; Q9H4A7
• Molecular Weight: 20,121 Da
• NCBI Official Full Name: vesicle-associated membrane protein 7 isoform 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 7
• NCBI Official Symbol: VAMP7
• NCBI Official Synonym Symbols: SYBL1; TIVAMP; VAMP-7; TI-VAMP
• NCBI Protein Information: vesicle-associated membrane protein 7; synaptobrevin-like 1; synaptobrevin-like protein 1; tetanus neurotoxin-insensitive VAMP; tetanus-insensitive VAMP
• UniProt Protein Name: Vesicle-associated membrane protein 7
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP7
• UniProt Synonym Gene Names: SYBL1; VAMP-7; Ti-VAMP
• UniProt Entry Name: VAMP7_HUMAN

NCBI Description

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

VAMP7: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28 and Yq12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; neuron projection; cell surface; intracellular membrane-bound organelle; late endosome membrane; secretory granule membrane; lysosomal membrane; integral to membrane; trans-Golgi network; phagocytic vesicle; pseudopodium; secretory granule; SNARE complex; phagocytic vesicle membrane; membrane; perinuclear region of cytoplasm; apical part of cell; lamellipodium; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: SNAP receptor activity; protein binding; SNARE binding; syntaxin binding

Biological Process: ER to Golgi vesicle-mediated transport; exocytosis; autophagic vacuole fusion; natural killer cell degranulation; neutrophil degranulation; vesicle fusion with Golgi apparatus; endocytosis; calcium ion-dependent exocytosis; vesicle-mediated transport; vesicle fusion; Golgi to plasma membrane protein transport; endosome to lysosome transport; eosinophil degranulation; post-Golgi vesicle-mediated transport; triacylglycerol transport; phagocytosis, engulfment