Human Midkine ELISA Kit | MDK elisa kit

Midkine (Human) OmniKine ELISA Kit

Cat. Num. AAA9501876

• Gene Names: MDK; MK; ARAP; NEGF2
• Reactivity: Human
• Synonyms: Midkine; Midkine (Human) OmniKine ELISA Kit; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein; MK1; NEGF2; MDK elisa kit

• Reactivity: Human
• Specificity: The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 143

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human Midkine ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Midkine proteins within the range of 32-2000 pg/ml.)

Related Product Information for MDK elisa kit

Principle of the Assay: The OmniKine™ Human Midkine ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Midkine concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Midkine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human Midkine, also known as Amphiregulin-Associated Protein, is a 143 amino acid cytokine protein encoded by the MDK gene located at locus 11p11.2 on chromosome 11. After initial synthesis, the protein undergoes proteolytic processing to remove the 20 residue signal sequence from the 123 residue Midkine peptide, allowing the protein to properly fold and mature. Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis that promotes angiogenesis, cell growth, and cell migration. Human Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis. Belonging to the pleiotrophin family, Midkine is also involved in early fetal adrenal gland development as well as neointima formation after arterial injury, possibly by mediating leukocyte recruitment.

NCBI and Uniprot Product Information

• NCBI GI #: 59894796
• NCBI GeneID: 4192
• NCBI Accession #: NP_001012333.1
• NCBI GenBank Nucleotide #: NM_001012333.2
• UniProt Accession #: P21741; Q2LEK4; Q9UCC7
• Molecular Weight: 9,512 Da
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: MDK
• NCBI Official Synonym Symbols: MK; ARAP; NEGF2
• NCBI Protein Information: midkine
• UniProt Protein Name: Midkine
• Protein Family: Midkine
• UniProt Gene Name: MDK
• UniProt Synonym Gene Names: MK1; NEGF2; MK; ARAP

NCBI Description

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: growth factor activity; heparin binding

Biological Process: adrenal gland development; behavioral fear response; cell differentiation; cell migration; cerebellar granular layer development; cerebral cortex development; defecation; dentate gyrus development; negative regulation of neuron apoptosis; nervous system development; Notch signaling pathway; positive regulation of cell division; positive regulation of transcription, DNA-templated; regulation of behavior; response to drug; response to glucocorticoid stimulus; response to wounding; short-term memory; signal transduction