Human MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit | MCP-3 clia kit

Human MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit

Cat. Num. AAA2533151

• Reactivity: Human
• Synonyms: MCP-3 (Monocyte Chemotactic Protein 3); Human MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit; MCP3; FIC; MARC; NC28; SCYA6; SCYA7; MCP-3 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant MCP-3. No significant cross-reactivity or interference between MCP-3 and analogues was observed.
• Sequence Length: 109

• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MCP-3 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of MCP-3 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to MCP-3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of MCP-3. You can calculate the concentration of MCP-3 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 313708
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: monocyte chemotactic protein-3
• UniProt Protein Name: C-C motif chemokine 7
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; cell; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; protein binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: neutrophil chemotaxis; cytoskeleton organization and biogenesis; signal transduction; chemotaxis; cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; G-protein coupled receptor protein signaling pathway; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; inflammatory response; positive regulation of GTPase activity; positive regulation of cell migration