Chicken Myoglobin ELISA Kit | MB elisa kit

Chicken Myoglobin ELISA Kit

• Gene Names: MB; PVALB
• Reactivity: Chicken
• Synonyms: Myoglobin; Chicken Myoglobin ELISA Kit; MB elisa kit

• Reactivity: Chicken
• Specificity: No significant cross-reactivity or interference between Chicken MB and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MB concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken MB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MB and MB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MB elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MB concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken MB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MB and MB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312.1
• NCBI GenBank Nucleotide #: NM_203378.1
• UniProt Accession #: P02144; Q52H51; Q5THY7
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; response to hypoxia; brown fat cell differentiation; enucleate erythrocyte differentiation