Bovine TGF-beta (Transforming Growth Factor Beta) ELISA Kit | BMP7 elisa kit

Bovine TGF-beta (Transforming Growth Factor Beta)

Cat. Num. AAA766055

• Gene Names: BMP7; OP-1
• Reactivity: Bovine
• Synonyms: TGF-beta (Transforming Growth Factor Beta); Bovine TGF-beta (Transforming Growth Factor Beta); BMP7 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of TGF-beta. No significant cross-reactivity or interference between TGF-beta and analogues was observed.
• Sequence Length: 431

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 339564
• NCBI GeneID: 655
• Molecular Weight: 49,313 Da
• NCBI Official Full Name: transforming growth factor-beta
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: BMP7
• NCBI Official Synonym Symbols: OP-1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP7
• UniProt Synonym Gene Names: OP1; BMP-7; OP-1
• UniProt Entry Name: BMP7_HUMAN

NCBI Description

This gene encodes a member of the transforming growth factor-beta (TGF-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate each subunit of the mature homodimer, which plays a role in bone, kidney and brown adipose tissue development. This protein induces ectopic bone formation and may promote fracture healing in human patients. [provided by RefSeq, Nov 2015]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; heparin binding; protein binding; transforming growth factor beta receptor binding

Biological Process: axon guidance; BMP signaling pathway; branching morphogenesis of a tube; cartilage development; dendrite development; embryonic camera-type eye morphogenesis; embryonic limb morphogenesis; embryonic pattern specification; epithelial cell differentiation; epithelial to mesenchymal transition; growth; inhibition of NF-kappaB transcription factor; mesenchymal cell differentiation; mesoderm formation; mesonephros development; metanephros development; negative regulation of cell cycle; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of neuron differentiation; negative regulation of NF-kappaB import into nucleus; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; neurite morphogenesis; odontogenesis of dentine-containing teeth; ossification; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of heterotypic cell-cell adhesion; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; response to estradiol stimulus; response to peptide hormone stimulus; response to vitamin D; skeletal development; steroid hormone mediated signaling; ureteric bud development