Nectin-4 (NECTIN4) Active Protein | NECTIN4 active protein

Recombinant Human Nectin-4 (NECTIN4), Partial

Cat. Num. AAA7115154

• Gene Names: NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nectin-4 (NECTIN4); Recombinant Human Nectin-4 (NECTIN4); Partial; PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR; NECTIN4 active protein

• Host: Mammalian Cell
• Specificity: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum Filtered 20 mM PB, 150 mM NaCl, pH 7.4
• Sequence Positions: 31-351aa; Partial
• Sequence: AGELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV
• Sequence Length: 510

• Species: Human
• Tag: C-terminal 6xHis-tagged
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Biological Activity: Specific activity as determined by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells is greater than 40%. When 5 x 10^4 cells/well are added to plates coated with recombinant human Nectin-4 (1 ug/mL with 100 uL/well), approximately >68.8% will adhere after 40 min incubation at 37 degree C.
• Subcellular Location: Cell Membrane, Single-Pass Type I Membrane Protein, Cell Junction, adherens Junction, Note=Colocalizes with AFDN at cadherin-based adherens Junctions (PubMed:11544254), SUBCELLULAR LOCATION: Processed poliovirus receptor-related protein 4: Secreted
• Protein Families: Nectin Family
• Classification: Other Recombinant Protein
• Pathway: Adherens Junction
• Preparation and Storage: Store at -20 degree C. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for NECTIN4 active protein

Relevance: Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

Function: Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

Product Categories/Family for NECTIN4 active protein

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 222136611
• NCBI GeneID: 81607
• NCBI Accession #: NP_112178.2
• NCBI GenBank Nucleotide #: NM_030916.3
• UniProt Accession #: Q96NY8
• Molecular Weight: 35.3 kDa
• NCBI Official Full Name: nectin-4
• NCBI Official Synonym Full Names: nectin cell adhesion molecule 4
• NCBI Official Symbol: NECTIN4
• NCBI Official Synonym Symbols: LNIR; PRR4; EDSS1; PVRL4; nectin-4
• NCBI Protein Information: nectin-4
• UniProt Protein Name: Poliovirus receptor-related protein 4
• Protein Family: Nectin
• UniProt Gene Name: PVRL4
• UniProt Synonym Gene Names: LNIR; PRR4
• UniProt Entry Name: PVRL4_HUMAN

NCBI Description

This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]

Uniprot Description

nectin 4: Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. Defects in PVRL4 are the cause of ectodermal dysplasia- syndactyly syndrome type 1 (EDSS1). EDSS1 is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSS1 is characterized by the association of hair and teeth abnormalities with cutaneous syndactyly of the hands and/or feet. Hair morphologic abnormalities include twists at irregular intervals (pilli torti) and swelling along the shafts, particularly associated with areas of breakage. Dental findings consist of abnormally widely spaced teeth, with peg-shaped and conical crowns. Patients have normal sweating. Belongs to the nectin family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: adherens junction; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: intercellular junction assembly and maintenance; viral reproduction; cell adhesion

Disease: Ectodermal Dysplasia-syndactyly Syndrome 1