DFFA recombinant protein

DFFA, 1-331aa, Human, His tag, E Coli

Cat. Num. AAA203369

• Gene Names: DFFA; DFF1; ICAD; DFF-45
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DFFA; 1-331aa; Human; His tag; E Coli; DNA fragmentation factor subunit alpha.; FF-45; DFF1; ICAD; DNA fragmentation factor subunit alpha; A330085O09Rik; ICAD L; ICAD S; DFF 1; Caspase activated deoxyribonuclease inhibitor short form; DFF 45; DFF alpha; DFF35; DFF45; Caspase-activated deoxyribonuclease inhibitor short form; Dffa DNA fragmentation factor; alpha subunit; DNA fragmentation factor 45 kDa subunit; DNA Fragmentation Factor Alpha Subunit; DNA fragmentation factor; 45 kD; 45kDa; alpha polypeptide; alpha polypeptide (DFFA); transcript variant 1; RP23 121D17.3; DNAation factor 45 kDa subunit; H13; Inhibitor of CAD; Inhibitor of Caspase Activated DNase; MGC143066; OTTHuMP00000001903; OTTHuMP00000001904; ; DFFA recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T
• Sequence Length: 331

• Applicable Applications for DFFA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DFFA recombinant protein

DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DFFA recombinant protein

Apoptosis

References

McCarty JS., et al. (1999) Biochem Biophys Res Commun. 264 (1):176-80. ; Gu J., et al. (1999) J Biol Chem. 274 (30):20759-62.;

NCBI and Uniprot Product Information

• NCBI GI #: 14043461
• NCBI GeneID: 1676
• Molecular Weight: 38.7 kDa (351aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• NCBI Official Synonym Full Names: DNA fragmentation factor subunit alpha
• NCBI Official Symbol: DFFA
• NCBI Official Synonym Symbols: DFF1; ICAD; DFF-45
• NCBI Protein Information: DNA fragmentation factor subunit alpha
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: DFFA
• UniProt Synonym Gene Names: DFF1; DFF45; DFF-45; ICAD

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitor of the caspase-activated DNase (DFF40).