Human Metastasis-associated in colon cancer protein 1 ELISA Kit | MACC1 elisa kit

Human Metastasis-associated in colon cancer protein 1 ELISA Kit

Cat. Num. AAA762824

• Gene Names: MACC1; 7A5; SH3BP4L
• Reactivity: Human
• Synonyms: Metastasis-associated in colon cancer protein 1; Human Metastasis-associated in colon cancer protein 1 ELISA Kit; MACC1 (Metastasis-associated in colon cancer protein 1)/SH3 domain-containing protein 7a5; MACC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MACC1. No significant cross-reactivity or interference between MACC1 and analogues was observed.
• Sequence Length: 852

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MACC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MACC1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MACC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MACC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MACC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MACC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MACC1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157502191
• NCBI GeneID: 346389
• NCBI Accession #: NP_877439.3
• NCBI GenBank Nucleotide #: NM_182762.3
• Molecular Weight: 96,639 Da
• NCBI Official Full Name: metastasis-associated in colon cancer protein 1
• NCBI Official Synonym Full Names: metastasis associated in colon cancer 1
• NCBI Official Symbol: MACC1
• NCBI Official Synonym Symbols: 7A5; SH3BP4L
• NCBI Protein Information: metastasis-associated in colon cancer protein 1
• UniProt Protein Name: Metastasis-associated in colon cancer protein 1
• Protein Family: Metastasis-associated in colon cancer protein
• UniProt Gene Name: MACC1
• UniProt Entry Name: MACC1_HUMAN

NCBI Description

MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).[supplied by OMIM, Mar 2009]

Uniprot Description

MACC1: Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: cytoplasm; mitochondrion; nucleus

Molecular Function: growth factor activity

Biological Process: positive regulation of cell division; regulation of transcription, DNA-dependent; transcription, DNA-dependent