Rat ELAV1 (ELAV-like protein 1) ELISA Kit | ELAV1 elisa kit

Rat ELAV1 (ELAV-like protein 1) ELISA Kit

Cat. Num. AAA7608250

• Gene Names: Elavl1; HuR; Hua
• Reactivity: Rat
• Synonyms: ELAV1 (ELAV-like protein 1); Rat ELAV1 (ELAV-like protein 1) ELISA Kit; ELAV like protein 1; ELAV1; ELAVL1; Hu antigen R; Hua; HuR; MelG; ELAV1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ELAV1. No significant cross-reactivity or interference between ELAV1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELAV1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157818153
• NCBI GeneID: 363854
• NCBI Accession #: NP_001102318.1
• NCBI GenBank Nucleotide #: NM_001108848.1
• UniProt Accession #: B5DF91
• Molecular Weight: 36,169 Da
• NCBI Official Full Name: ELAV-like protein 1
• NCBI Official Synonym Full Names: ELAV like RNA binding protein 1
• NCBI Official Symbol: Elavl1
• NCBI Official Synonym Symbols: HuR; Hua
• NCBI Protein Information: ELAV-like protein 1
• UniProt Protein Name: ELAV-like protein 1
• UniProt Gene Name: Elavl1
• UniProt Synonym Gene Names: HuR

NCBI Description

RNA binding protein; involved in posttranscriptional regulation of VEGF expression [RGD, Feb 2006]

Uniprot Description

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA ().