Human metastasis associated in colon cancer 1 ELISA Kit | MACC1 elisa kit

Human Metastasis-associated in colon cancer protein 1, MACC1 ELISA Kit

Cat. Num. AAA920839

• Gene Names: MACC1; 7A5; SH3BP4L
• Reactivity: Human
• Synonyms: metastasis associated in colon cancer 1; Human Metastasis-associated in colon cancer protein 1; MACC1 ELISA Kit; Human Metastasis-associated in colon cancer protein 1 (MACC1) ELISA kit; 7A5; SH3BP4L; OTTHUMP00000201891; putative binding protein 7a5; MACC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human M2-PK. No significant cross-reactivity or interference between human M2-PK and analogues was observed.
• Sequence Length: 852

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.9 U/ml-60 U/ml
• Sensitivity: 0.225 U/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MACC1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for M2-PK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M2-PK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M2-PK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M2-PK bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 157502191
• NCBI GeneID: 346389
• NCBI Accession #: NP_877439.3
• NCBI GenBank Nucleotide #: NM_182762.3
• UniProt Accession #: Q6ZN28; Q6ZQN8; Q7Z5A5; A8MUS5; B2RNR9
• Molecular Weight: 96,639 Da
• NCBI Official Full Name: metastasis-associated in colon cancer protein 1
• NCBI Official Synonym Full Names: metastasis associated in colon cancer 1
• NCBI Official Symbol: MACC1
• NCBI Official Synonym Symbols: 7A5; SH3BP4L
• NCBI Protein Information: metastasis-associated in colon cancer protein 1; putative binding protein 7a5; SH3 domain-containing protein 7a5
• UniProt Protein Name: Metastasis-associated in colon cancer protein 1
• Protein Family: Metastasis-associated in colon cancer protein
• UniProt Gene Name: MACC1
• UniProt Entry Name: MACC1_HUMAN

NCBI Description

MACC1 is a key regulator of the hepatocyte growth factor (HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed 19098908]).[supplied by OMIM, Mar 2009]

Uniprot Description

MACC1: Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: growth factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell division