Lumican Recombinant Protein | LUM recombinant protein

Recombinant Lumican

Cat. Num. AAA201136

• Gene Names: Lum; Ldc; SLRR2D
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Lumican; Recombinant Lumican; Lum; Ldc; SLRR2D; Keratan sulfate proteoglycan lumican; KSPG lumican; LUM recombinant protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 20% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 19-338aa
• Sequence: QYYDYDIPLF MYGQISPNCA PECNCPHSYP TAMYCDDLKL KSVPMVPPGI KYLYLRNNQI DHIDEKAFEN VTDLQWLILD LLENSKIK GKVFSKLKQL KKLHINYNNL TESVGPLPKS LQDLQLTNNK ISKLGSFDGL VNLTFIYLQH NQLKEDAVSA SLKGLKSLEY LDLSFNQMSK LPAGLPTSLL TLYLDNNKIS NIPDEYFKRF TGLQYLRLSH NELADSGVPG NSFNISSLLE LDLSYNKLKS IPTVNENLEN YYLEVNELEK FDVKSFCKIL GPLSYSKIKH LRLDGNPLTQ SSLPPDMYEC LRVANEITVN VEHHHHHH
• Sequence Length: 338

• Applicable Applications for LUM recombinant protein: SDS-PAGE
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Gln 19
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for LUM recombinant protein

Lumican, also known as Lum, is a proteoglycan Class II member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. It is a major component of the corna, demal, and muscle connective tissues. This protein has a negatively-charged N-terminal domain containing sulfated tyrosine and disulfide bonds and ten tandem leucine-rich that repeats allowing it to bind to other extracellular components such as collagen. Lum is the major keratin sulfate proteoglycan of the cornea and is distributed in interstitial collagenous matrices throughout the body. Also, it plays a role as definitive link between a necessity for this protein in the development of a highly organized collagenous matrix and corneal transparency. Recombinant Mouse Lumican, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for LUM recombinant protein

Cancer

References

Castillo EF., et al, (2016) Eur. J. Immunol. 46:2852-2861. Ying S., et al, (1997) J. Biol. Chem. 272:30306-30313.

NCBI and Uniprot Product Information

• NCBI GI #: 21542415
• NCBI GeneID: 17022
• UniProt Accession #: P51885
• Molecular Weight: 37.5kDa (328aa); 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: Lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: Lum
• NCBI Official Synonym Symbols: Ldc; SLRR2D
• NCBI Protein Information: lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: Lum
• UniProt Synonym Gene Names: Lcn; Ldc; KSPG lumican
• UniProt Entry Name: LUM_MOUSE

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; fibrillar collagen; extracellular region

Molecular Function: collagen binding

Biological Process: collagen fibril organization; visual perception; positive regulation of transcription from RNA polymerase II promoter