Human Lumican ELISA Kit | LUM elisa kit

Human Lumican PicoKine ELISA Kit

Cat. Num. AAA177383

• Gene Names: LUM; LDC; SLRR2D
• Reactivity: Human
• Synonyms: Lumican; Human Lumican PicoKine ELISA Kit; lumican; Keratan sulfate proteoglycan lumican; KSPG Lumican; LDC; LUM; LUM_HUMAN; Lumican precursor; SLRR2D; LUM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human LUM
• Sequence Length: 338

• Samples: Cell culture supernatants, serum and plasma (heparin)
• Detection Range: 156 pg/ml - 10,000 pg/ml
• Sensitivity: <10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for LUM elisa kit

Principle of the Assay: The MBS Picokine™ Human LUM Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human LUM with a 96-well strip plate that is pre-coated with antibody specific for LUM. The detection antibody is a biotinylated antibody specific for LUM. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LUM with immunogen: Expression system for standard: NS0; Immunogen sequence: Q19-N338. The kit is analytically validated with ready-to-use reagents. To measure Human LUM, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human LUM in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human LUM in the sample.

Background/Introduction: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

NCBI and Uniprot Product Information

• NCBI GI #: 20141464
• NCBI GeneID: 4060
• UniProt Accession #: P51884; Q96QM7; B2R6R5
• Molecular Weight: 38,429 Da
• NCBI Official Full Name: Lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: LUM
• NCBI Official Synonym Symbols: LDC; SLRR2D
• NCBI Protein Information: lumican; KSPG lumican; lumican proteoglycan; keratan sulfate proteoglycan lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: LUM
• UniProt Synonym Gene Names: LDC; SLRR2D; KSPG lumican
• UniProt Entry Name: LUM_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; fibrillar collagen; Golgi lumen; extracellular region

Molecular Function: collagen binding; protein binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; extracellular matrix organization and biogenesis; collagen fibril organization; visual perception; glycosaminoglycan metabolic process; cartilage development; keratan sulfate biosynthetic process; carbohydrate metabolic process; positive regulation of transcription from RNA polymerase II promoter; pathogenesis; keratan sulfate catabolic process; response to organic cyclic substance