Bovine C-C motif chemokine 5, CCL5 ELISA Kit | CCL5 elisa kit

Bovine C-C motif chemokine 5, CCL5 ELISA Kit

Cat. Num. AAA1605191

• Gene Names: CCL5; RANTES
• Reactivity: Bovine
• Synonyms: C-C motif chemokine 5; CCL5; Bovine C-C motif chemokine 5; CCL5 ELISA Kit; CCL5 elisa kit

• Reactivity: Bovine
• Sequence Length: 91

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 2-600ng/mL
• Sensitivity: 1.27ng/mL
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CCL5 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Bovine C-C motif chemokine 5 (also known as CCL5) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine CCL5 antibody. CCL5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CCL5 Antibody is added and binds to CCL5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine CCL5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 126352606
• NCBI GeneID: 100033925
• NCBI GenBank Nucleotide #: NP_001075332.1
• UniProt Accession #: Q8MKD0
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Symbol: CCL5
• NCBI Official Synonym Symbols: RANTES
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL5
• UniProt Synonym Gene Names: SCYA5

Uniprot Description

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.