Typical Testing Data/Standard Curve (for reference only)
Rat Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit | MYBPC3 elisa kit
Rat Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit
Reactivity
Rat
Synonyms
Myosin Binding Protein C; Cardiac (MYBPC3); Rat Myosin Binding Protein C; Cardiac (MYBPC3) ELISA Kit; MYBPC-3; FHC; CMH4; MYBP-C; C-protein; cardiac muscle isoform; MYBPC3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of MYBPC3. No significant cross-reactivity or interference between MYBPC3 and analogues was observed.
Sequence Length
1274
Samples
Serum, plasma or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
<29.8pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYBPC3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYBPC3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MYBPC3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYBPC3 in rat serum, plasma or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYBPC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYBPC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYBPC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYBPC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYBPC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYBPC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYBPC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYBPC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
140,762 Da
NCBI Official Full Name
myosin-binding protein C, cardiac-type
NCBI Official Synonym Full Names
myosin binding protein C, cardiac
NCBI Official Symbol
Mybpc3
NCBI Protein Information
myosin-binding protein C, cardiac-type
UniProt Protein Name
Myosin-binding protein C, cardiac-type
Protein Family
UniProt Gene Name
Mybpc3
UniProt Synonym Gene Names
Cardiac MyBP-C
Uniprot Description
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Research Articles on MYBPC3
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Product Notes
The Rat MYBPC3 mybpc3 (Catalog #AAA457081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457081 ELISA Kit recognizes Rat MYBPC3. It is sometimes possible for the material contained within the vial of "Myosin Binding Protein C, Cardiac (MYBPC3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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