Human Low-density lipoprotein receptor-related protein 2 ELISA Kit | LRP2 elisa kit

Human Low-density lipoprotein receptor-related protein 2 ELISA Kit

Cat. Num. AAA763550

• Gene Names: Lrp2; Gp330; Megalin; AI315343; AW536255; b2b1625.2Clo; D230004K18Rik
• Reactivity: Human
• Synonyms: Low-density lipoprotein receptor-related protein 2; Human Low-density lipoprotein receptor-related protein 2 ELISA Kit; LRP2/gp330/Megalin/LRP-2/Low-density lipoprotein receptor-related protein 2/Glycoprotein 330; LRP2 elisa kit

• Reactivity: Human
• Sequence Length: 4660

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LRP2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human LRP2antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LRP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LRP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman LRP2can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 124487372
• NCBI GeneID: 14725
• NCBI Accession #: NP_001074557.1
• NCBI GenBank Nucleotide #: NM_001081088.1
• Molecular Weight: 519,208 Da
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 2
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 2
• NCBI Official Symbol: Lrp2
• NCBI Official Synonym Symbols: Gp330; Megalin; AI315343; AW536255; b2b1625.2Clo; D230004K18Rik
• NCBI Protein Information: low-density lipoprotein receptor-related protein 2
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 2
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: Lrp2
• UniProt Synonym Gene Names: LRP-2; gp330
• UniProt Entry Name: LRP2_MOUSE

Uniprot Description

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Cellular Component: apical part of cell; apical plasma membrane; brush border; brush border membrane; coated pit; cytoplasm; endocytic vesicle; endoplasmic reticulum; endosome; extracellular space; Golgi apparatus; integral to membrane; lipid raft; lysosomal membrane; membrane; protein complex; receptor complex

Molecular Function: calcium ion binding; hemoglobin binding; hormone binding; lipoprotein transporter activity; low-density lipoprotein receptor binding; metal ion binding; PDZ domain binding; protein binding; protein complex binding; receptor activity; SH3 domain binding

Biological Process: cell proliferation; endocytosis; endosome transport; forebrain development; heart development; hemoglobin import; hormone secretion; lipoprotein transport; receptor-mediated endocytosis; response to X-ray; transcytosis; vitamin metabolic process