Mouse Lysophosphatidic acid receptor 1 ELISA Kit | Lpar1 elisa kit

Mouse Lysophosphatidic acid receptor 1 ELISA Kit

Cat. Num. AAA9428861

• Gene Names: Lpar1; Edg2; Kdt2; lpA1; vzg-1; Gpcr26; AI326300
• Reactivity: Mouse
• Synonyms: Lysophosphatidic acid receptor 1; Mouse Lysophosphatidic acid receptor 1 ELISA Kit; LPA receptor 1; LPA-1; Lysophosphatidic acid receptor Edg-2; Rec1.3; VZG-1; Lpar1; Edg2; Gpcr26; Lpa1; Vzg1; Lpar1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouseGlycosylated hemoglobin A1c. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma,Tissue Homogenates And Cell Culture Supernates And Other Biological Fluids
• Detection Range: 3.12ng/ml-200ng/ml.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lpar1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofmouseGlycosylated hemoglobin A1cconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylated hemoglobin A1c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forGlycosylated hemoglobin A1c and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycosylated hemoglobin A1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Glycosylated hemoglobin A1c in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 594191040
• NCBI GeneID: 14745
• NCBI Accession #: NP_001277415.1
• NCBI GenBank Nucleotide #: NM_001290486.1
• UniProt Accession #: P61793; O88584; P56487; P70420; Q61130; A2AMJ2
• Molecular Weight: 39,343 Da
• NCBI Official Full Name: lysophosphatidic acid receptor 1 isoform 2
• NCBI Official Synonym Full Names: lysophosphatidic acid receptor 1
• NCBI Official Symbol: Lpar1
• NCBI Official Synonym Symbols: Edg2; Kdt2; lpA1; vzg-1; Gpcr26; AI326300
• NCBI Protein Information: lysophosphatidic acid receptor 1
• UniProt Protein Name: Lysophosphatidic acid receptor 1
• Protein Family: Lysophosphatidic acid receptor
• UniProt Gene Name: Lpar1
• UniProt Synonym Gene Names: LPA receptor 1Curated; LPA-1

Uniprot Description

LPAR1: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4 B3|4 32.2 cM

Cellular Component: cell soma; cell surface; cytoplasm; dendritic shaft; dendritic spine; endocytic vesicle; endosome; integral to plasma membrane; neuron projection; plasma membrane

Molecular Function: G-protein alpha-subunit binding; PDZ domain binding; phospholipid binding; protein binding

Biological Process: activation of MAPK activity; bleb formation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; negative regulation of cAMP biosynthetic process; phospholipase C activation; positive regulation of apoptosis; positive regulation of MAPKKK cascade; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; regulation of cell shape