Human Lysophosphatidic Acid Receptor 1 (LPAR1) ELISA Kit | LPAR1 elisa kit

Human Lysophosphatidic Acid Receptor 1 (LPAR1) ELISA Kit

Cat. Num. AAA2022229

• Gene Names: LPAR1; EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3
• Reactivity: Human
• Synonyms: Lysophosphatidic Acid Receptor 1 (LPAR1); Human Lysophosphatidic Acid Receptor 1 (LPAR1) ELISA Kit; EDG2; Gpcr26; LPA1; Mrec1.3; edg-2; rec.1.3; vzg-1; Endothelial Differentiation; Lysophosphatidic Acid G-Protein-Coupled Receptor 2; LPAR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LPAR1. No significant cross-reactivity or interference between LPAR1 and analogues was observed.
• Sequence Length: 365

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LPAR1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LPAR1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPAR1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LPAR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LPAR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LPAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LPAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LPAR1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 16950638
• NCBI GeneID: 1902
• NCBI Accession #: NP_476500.1
• NCBI GenBank Nucleotide #: NM_057159.2
• UniProt Accession #: Q92633; O00656; O00722; P78351; B4DK36
• Molecular Weight: 41,346 Da
• NCBI Official Full Name: lysophosphatidic acid receptor 1
• NCBI Official Synonym Full Names: lysophosphatidic acid receptor 1
• NCBI Official Symbol: LPAR1
• NCBI Official Synonym Symbols: EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3
• NCBI Protein Information: lysophosphatidic acid receptor 1
• UniProt Protein Name: Lysophosphatidic acid receptor 1
• Protein Family: Lysophosphatidic acid receptor
• UniProt Gene Name: LPAR1
• UniProt Synonym Gene Names: EDG2; LPA1; LPA receptor 1; LPA-1
• UniProt Entry Name: LPAR1_HUMAN

NCBI Description

The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul 2008]

Uniprot Description

LPAR1: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q31.3

Cellular Component: cell surface; integral to plasma membrane; endocytic vesicle; plasma membrane; dendritic spine; dendritic shaft

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; phospholipid binding; PDZ domain binding

Biological Process: myelination; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; activation of MAPK activity; phospholipase C activation; positive regulation of Rho protein signal transduction; bleb formation