Human 12 Lipoxygenase ELISA Kit | LOX 12 elisa kit
Human 12 Lipoxygenase ELISA Kit
Principle of the Assay||LOX12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LOX12. Standards or samples are then added to the microtiter plate wells and LOX12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LOX12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LOX12 are added to each well to "sandwich" the LOX12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LOX12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX12 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.33; Lipid Metabolism - linoleic acid; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.31; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol
Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding
Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance; bone mineralization
Research Articles on LOX 12
Similar Products
Product Notes
The Human LOX 12 alox15 (Catalog #AAA730723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730723 ELISA Kit recognizes Human LOX 12. It is sometimes possible for the material contained within the vial of "12 Lipoxygenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
