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Western Blot (WB)
(PAICS Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAICS antibody detected the PAICS protein (arrow).)
Rabbit anti-Human, Mouse PAICS Polyclonal Antibody | anti-PAICS antibody
PAICS, NT (PAICS, ADE2, AIRC, PAIS, Multifunctional protein ADE2, Phosphoribosylaminoimidazole-succinocarboxamide synthase, SAICAR synthetase, Phosphoribosylaminoimidazole carboxylase, AIR carboxylase) (FITC)
Gene Names
PAICS; ADE2; AIRC; PAIS; ADE2H1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PAICS; Polyclonal Antibody; NT (PAICS; ADE2; AIRC; PAIS; Multifunctional protein ADE2; Phosphoribosylaminoimidazole-succinocarboxamide synthase; SAICAR synthetase; Phosphoribosylaminoimidazole carboxylase; AIR carboxylase) (FITC); AIR carboxylase; anti-PAICS antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-PAICS antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PAICS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the N-terminal region of human PAICS.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PAICS Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAICS antibody detected the PAICS protein (arrow).)
Western Blot (WB)
(PAICS Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAICS antibody detected the PAICS protein (arrow).)
Western Blot (WB)
(PAICS Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PAICS antibody detected the PAICS protein (arrow).)
Western Blot (WB)
(PAICS Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the PAICS antibody detected the PAICS protein (arrow).)
Immunohistochemistry (IHC)
(PAICS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAICS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PAICS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAICS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PAICS Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PAICS Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PAICS antibody
This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-PAICS antibody
References
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010). Ikeda, M., et al. Biol. Psychiatry 67(3):263-269(2010). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,079 Da
NCBI Official Full Name
multifunctional protein ADE2 isoform 2
NCBI Official Synonym Full Names
phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
NCBI Official Symbol
PAICS
NCBI Official Synonym Symbols
ADE2; AIRC; PAIS; ADE2H1
NCBI Protein Information
multifunctional protein ADE2; AIR carboxylase; SAICAR synthetase; multifunctional protein ADE2H1
UniProt Protein Name
Multifunctional protein ADE2
Protein Family
UniProt Gene Name
PAICS
UniProt Synonym Gene Names
ADE2; AIRC; PAIS; AIRC
Similar Products
Product Notes
The PAICS paics (Catalog #AAA6330530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAICS, NT (PAICS, ADE2, AIRC, PAIS, Multifunctional protein ADE2, Phosphoribosylaminoimidazole-succinocarboxamide synthase, SAICAR synthetase, Phosphoribosylaminoimidazole carboxylase, AIR carboxylase) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PAICS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PAICS paics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAICS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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