Human Farnesyl Diphosphate Synthase (FDPS) ELISA Kit | FDPS elisa kit

Human Farnesyl Diphosphate Synthase (FDPS) ELISA Kit

Cat. Num. AAA2705390

• Gene Names: FDPS; FPS; FPPS; POROK9
• Reactivity: Human
• Synonyms: Farnesyl Diphosphate Synthase (FDPS); Human Farnesyl Diphosphate Synthase (FDPS) ELISA Kit; FPS; Farnesyl Pyrophosphate Synthetase; Dimethylallyltranstransferase; Geranyltranstransferase; FDPS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Farnesyl Diphosphate Synthase (FDPS).; No significant cross-reactivity or interference between Farnesyl Diphosphate Synthase (FDPS) and analogues was observed.
• Sequence Length: 353

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: < 26.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FDPS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyl Diphosphate Synthase (FDPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyl Diphosphate Synthase (FDPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyl Diphosphate Synthase (FDPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesyl Diphosphate Synthase (FDPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 215274250
• NCBI GeneID: 2224
• UniProt Accession #: P14324; Q96G29; D3DV91; E9PCI9
• Molecular Weight: 40,532 Da
• NCBI Official Full Name: Farnesyl pyrophosphate synthase
• NCBI Official Synonym Full Names: farnesyl diphosphate synthase
• NCBI Official Symbol: FDPS
• NCBI Official Synonym Symbols: FPS; FPPS; POROK9
• NCBI Protein Information: farnesyl pyrophosphate synthase
• UniProt Protein Name: Farnesyl pyrophosphate synthase
• Protein Family: Farnesyl pyrophosphate synthase
• UniProt Gene Name: FDPS
• UniProt Synonym Gene Names: FPS; KIAA1293; FPP synthase; FPS

NCBI Description

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008]

Uniprot Description

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.