Mouse anti-Human ILT3 Monoclonal Antibody | anti-LILRB4 antibody

Anti-ILT3 Antibody (FITC), Mouse Monoclonal

Cat. Num. AAA8107759

• Gene Names: LILRB4; ILT3; LIR5; CD85K; ILT-3; LIR-5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: ILT3; Monoclonal Antibody; Anti-ILT3 Antibody (FITC); Mouse Monoclonal; LILRB4/CD85k/ILT3 Antibody (FITC); Mouse MAb; leukocyte immunoglobulin like receptor B4; Anti-CD85K Antibody; Anti-ILT-3 Antibody; Anti-ILT3 Antibody; Anti-LIR-5 Antibody; Anti-LIR5 Antibody; anti-LILRB4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 05
• Specificity: Human LILRB4/CD85k/ILT3
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-LILRB4 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human LILRB4/CD85k/ILT3 Protein
• Conjugation: FITC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LILRB4/CD85k/ILT3 (rh LILRB4/CD85k/ILT3) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human LILRB4(CD85k) expression on human whole blood monocytes. Cells were stained with FITC-conjugated anti-Human LILRB4(CD85k). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.)

Related Product Information for anti-LILRB4 antibody

LILRB4 (Leukocyte Immunoglobulin Like Receptor B4) is a Protein Coding gene. This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. LILRB4 is believed to down-regulate activation signals mediated by non-receptor tyrosine kinase cascades through the recruitment of SHP-1. LILRB4 is associated with the pathological processes of various inflammatory diseases. The LILRB4 is a central inhibitory receptor in Uterine dendritic cells (uDCs) that plays essential immune-regulatory roles at the maternal-fetal interface.

NCBI and Uniprot Product Information

• NCBI GI #: 618857488
• NCBI GeneID: 11006
• NCBI Accession #: NP_001265357.2
• NCBI GenBank Nucleotide #: NM_001278428.3
• UniProt Accession #: Q8NHJ6; O15468; O75021; Q6FGQ9; Q8N1C7; Q8NHL5; A8MVL8
• Molecular Weight: 49,397 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 4 isoform 3
• NCBI Official Synonym Full Names: leukocyte immunoglobulin like receptor B4
• NCBI Official Symbol: LILRB4
• NCBI Official Synonym Symbols: ILT3; LIR5; CD85K; ILT-3; LIR-5
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 4
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 4
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB4
• UniProt Synonym Gene Names: ILT3; LIR5; ILT-3; LIR-5
• UniProt Entry Name: LIRB4_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB4: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5- signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; receptor activity; antigen binding

Biological Process: negative regulation of osteoclast differentiation; immune system process; signal transduction