Legumain Recombinant Protein | LGMN recombinant protein

Recombinant Legumain (LGMN)

Cat. Num. AAA2029481

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Legumain; Recombinant Legumain (LGMN); LGMN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; VPI DDPEDGGKHW VVIVAGSNGW YNYRHQADAC HAYQIIHRNG IPDEQIVVMM YDDIAYSEDN PTPGIVINRP NGTDVYQGVP KDYTGEDVTP QNFLAVLRGD AEAVKGIGSG KVLKSGPQDH VFIYFTDHGS TGILVFPNED LHVKDLNETI HYMYKHKMYR KMVFYIEACE SGSMMNHLPD NINVYATTAA NPRESSYACY YDEKRSTYLG DWYSVNWMED SDVEDLTKET LHKQYHLVKS HTNTSHVMQY GNKTISTMKV MQFQGMKRKA SSPVPLPPVT HLDLTPSPDV PLTIMKRKLM NTNDLEESRQ LTEEIQRHLD ARHLIEKSVR KIVSLLAASE AEVEQLLSER APLTGHSCYP EALLHFRTHC FNWHSPTYEY ALRHLYVLVN LCEKPYPLHR IKLSMDHVCLGHY
• Sequence Length: 433

• Applicable Applications for LGMN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 51.4kDa
• Accurate Molecular Mass (KD): 51kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ile219~Met421
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 56682964
• NCBI GeneID: 5641
• NCBI Accession #: NP_001008530.1
• NCBI GenBank Nucleotide #: NM_001008530.2
• Molecular Weight: 42,008 Da
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• Protein Family: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1
• UniProt Entry Name: LGMN_HUMAN

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: Protease; EC 3.4.22.34

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: lysosomal lumen; apical part of cell; lysosome; late endosome

Molecular Function: peptidase activity; cysteine-type endopeptidase activity

Biological Process: steroid metabolic process; renal system process; negative regulation of multicellular organism growth; proteolysis involved in cellular protein catabolic process; receptor catabolic process; toll-like receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; innate immune response; negative regulation of neuron apoptosis; response to acidity; proteolysis; vitamin D metabolic process