Human Legumain ELISA Kit | LGMN elisa kit

Human Legumain(total) PicoKine ELISA Kit

Cat. Num. AAA178752

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Reactivity: Human
• Synonyms: Legumain; Human Legumain(total) PicoKine ELISA Kit; AEP; Asparaginyl endopeptidase; cysteine 1; Cysteine protease 1; EC 3.4.22.34; LGMN; LGMN_HUMAN; LGMN1; Protease; Protease cysteine 1; Protease cysteine 1 (legumain); PRSC1; Q99538; LGMN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Legumain(total)
• Sequence Length: 433

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 20-25
• Immunogen: NSO, V18-Y433
• Sample Data: Concentration(pg/ml): |0|31.2|62.5|125|250|500|1000|2000; O.D.: |0.007|0.151|0.203|0.307|0.508|0.871|1.413|1.988
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |41.2|167|841|55|201|755; Standard Deviation: |2.34|11.86|62.23|2.98|10.25|46.81; CV(%): |5.7|7.1|7.4|5.4|5.1|6.2
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Legumain(total) standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human Legumain(total) standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Legumain(total) PicoKine ELISA Kit standard curve )

Related Product Information for LGMN elisa kit

For quantitative detection of human Legumain(total) in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
Principle: Human Legumain(total) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Legumain(total) has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: V18-Y433) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Legumain(total) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Legumain(total) amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 56682964
• NCBI GeneID: 5641
• NCBI Accession #: NP_001008530.1
• NCBI GenBank Nucleotide #: NM_001008530.2
• UniProt Accession #: Q99538; O00123; Q86TV2; Q86TV3; Q9BTY1
• Molecular Weight: 42,008 Da
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• Protein Family: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: EC 3.4.22.34; Protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: lysosomal lumen; lysosome

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process