Rabbit anti-Human, Mouse ATP5F1 Polyclonal Antibody | anti-ATP5F1 antibody

ATP5F1 Polyclonal Antibody

Cat. Num. AAA3008804

• Gene Names: ATP5PB; PIG47; ATP5F1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP5F1; Polyclonal Antibody; ATP5F1 Polyclonal Antibody; PIG47; anti-ATP5F1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP5F1 polyclonal antibody detects endogenous levels of ATP5F1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 256

• Applicable Applications for anti-ATP5F1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human ATP5F1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using ATP5F1 antibody.)

Related Product Information for anti-ATP5F1 antibody

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

NCBI and Uniprot Product Information

• NCBI GI #: 20455474
• NCBI GeneID: 515
• UniProt Accession #: P24539
• Molecular Weight: ~ 29kDa
• NCBI Official Full Name: ATP synthase F(0) complex subunit B1, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase peripheral stalk-membrane subunit b
• NCBI Official Symbol: ATP5PB
• NCBI Official Synonym Symbols: PIG47; ATP5F1
• NCBI Protein Information: ATP synthase F(0) complex subunit B1, mitochondrial
• UniProt Protein Name: ATP synthase F(0) complex subunit B1, mitochondrial
• Protein Family: ATP synthase F(0) complex
• UniProt Gene Name: ATP5F1
• UniProt Synonym Gene Names: ATPase subunit b
• UniProt Entry Name: AT5F1_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5F1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Belongs to the eukaryotic ATPase B chain family.

Protein type: Hydrolase; Mitochondrial; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: nucleoplasm; mitochondrion; membrane; mitochondrial matrix; mitochondrial inner membrane; nucleus; mitochondrial proton-transporting ATP synthase complex

Molecular Function: protein binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity

Biological Process: cellular metabolic process; ATP synthesis coupled proton transport; substantia nigra development; mitochondrial ATP synthesis coupled proton transport