Lactate Dehydrogenase A (LDHA) Recombinant Protein | LDHA recombinant protein

Recombinant Lactate Dehydrogenase A (LDHA)

Cat. Num. AAA2011849

• Gene Names: Ldha; Ldh1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 97%
• Synonyms: Lactate Dehydrogenase A (LDHA); Recombinant Lactate Dehydrogenase A (LDHA); LDHA recombinant protein

• Host: E Coli
• Purity/Purification: > 97%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGSEF MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QF
• Sequence Length: 332

• Applicable Applications for LDHA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Phe332 (Accession # P04642) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8393706
• NCBI GeneID: 24533
• NCBI Accession #: NP_058721.1
• NCBI GenBank Nucleotide #: NM_017025.1
• UniProt Accession #: P04642
• Molecular Weight: 38kDa
• NCBI Official Full Name: L-lactate dehydrogenase A chain
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: Ldha
• NCBI Official Synonym Symbols: Ldh1
• NCBI Protein Information: L-lactate dehydrogenase A chain; LDH-A; LDH-M; LDH muscle subunit; lactate dehydrogenase A-like
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldha
• UniProt Synonym Gene Names: Ldh-1; Ldh1; LDH-A; LDH-M
• UniProt Entry Name: LDHA_RAT

NCBI Description

mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Apoptosis; Oxidoreductase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine; Carbohydrate Metabolism - pyruvate; EC 1.1.1.27

Cellular Component: mitochondrion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; lactate dehydrogenase activity; L-lactate dehydrogenase activity; kinase binding; NAD binding

Biological Process: response to drug; response to cAMP; positive regulation of apoptosis; response to organic cyclic substance; substantia nigra development; response to hydrogen peroxide; NAD metabolic process; response to estrogen stimulus; post-embryonic organ development; lactate metabolic process; response to glucose stimulus; cellular response to extracellular stimulus; response to hypoxia; cellular carbohydrate metabolic process; response to nutrient