Lactate Dehydrogenase A Recombinant Protein | LDHA recombinant protein

Recombinant Lactate Dehydrogenase A (LDHA)

Cat. Num. AAA2031773

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Lactate Dehydrogenase A; Recombinant Lactate Dehydrogenase A (LDHA); LDHA recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MATLKDQLIH NLLKEEHVPH NKITVVGVGA VGMACAISIL MKELADEIAL VDVMEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSR LVVITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPN CKLLVVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWIL GEHGDSSVPV WSGVNVAGVS LKNLHPELGT DADKEHWKAV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLSVPCILGQNGI SDVVKVTLTP EEEAHLKKSA DTLWGIQKEL QF
• Sequence Length: 12

• Applicable Applications for LDHA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 66.6kDa
• Accurate Molecular Mass (KD): 67kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Sus scrofa; Porcine (Pig)
• Fragment: Gln24~Leu100
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 239613
• Molecular Weight: 26,712 Da
• NCBI Official Full Name: lactate dehydrogenase A, partial
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHA
• UniProt Synonym Gene Names: LDH-A; LDH-M
• UniProt Entry Name: LDHA_HUMAN

Uniprot Description

LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - pyruvate; Oxidoreductase; EC 1.1.1.27; Apoptosis; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: mitochondrion; membrane; nucleus; cytosol

Molecular Function: protein binding; L-lactate dehydrogenase activity

Biological Process: cellular metabolic process; glycolysis; substantia nigra development; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process

Disease: Glycogen Storage Disease Xi