Ldha active protein

Ldha, 1-332aa, Mouse, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206245

• Gene Names: Ldha; Ldh1; Ldhm; l7R2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Ldha; 1-332aa; Mouse; His tag; Baculovirus (Bioactivity Validated); L-lactate dehydrogenase A chain isoform 1; 17R2; Ldh1; Ldhm; Ldha active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH
• Sequence Length: 332

• Applicable Applications for Ldha active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 250 units/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Ldha active protein

Ldha, also known as L-lactate dehydrogenase A chain isoform 1, is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It plays an important role in the development, invasion and metastasis of malignancies. Recombinant mouse Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Ldha active protein

Signal Transduction; Enzymes & Proteases

References

Rong Y., et al. (2013) Tumour Biol. 34 (3):1523-1530.; Merkle S., et al. (1992) Genetics. 131 (2):413-421.

NCBI and Uniprot Product Information

• NCBI GI #: 6754524
• NCBI GeneID: 16828
• NCBI Accession #: NP_034829
• NCBI GenBank Nucleotide #: NM_010699.2
• UniProt Accession #: P06151
• Molecular Weight: 37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: L-lactate dehydrogenase A chain isoform 1
• NCBI Official Synonym Full Names: lactate dehydrogenase A
• NCBI Official Symbol: Ldha
• NCBI Official Synonym Symbols: Ldh1; Ldhm; l7R2
• NCBI Protein Information: L-lactate dehydrogenase A chain
• UniProt Protein Name: L-lactate dehydrogenase A chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldha
• UniProt Synonym Gene Names: Ldh-1; Ldh1; LDH-A; LDH-M

NCBI Description

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to hemolytic anemia and early postimplantation death in mice. Multiple transcript variants encoding different isoforms have been found for this gene. The mouse genome contains multiple pseudogenes of this gene. [provided by RefSeq, May 2013]