Lipopolysaccharide Binding Protein (LBP) Recombinant Protein | LBP recombinant protein

Recombinant Lipopolysaccharide Binding Protein (LBP)

Cat. Num. AAA2010738

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lipopolysaccharide Binding Protein (LBP); Recombinant Lipopolysaccharide Binding Protein (LBP); LBP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-NPGL VVRITDQGLE YVAQEELLAL QSKLHKVTLP DFNGDVRIKH FGSVDYRFHS LNIQSCKLLG SALKLLPNQG LHFSISDSFI QVTGDWKVRK RILRLDGSFD VKVKGITISV NLLLDSEPSG RPKVAVSSCS SHIRDVEVHI SGDLGWLLNL FHNQIESRFR RVLESKICEI IEDSVTSELQ PYLQTLPVTT EIDHLAGLDY SLMGAPQATA QMLDVMFKGE IFSRDDRFPV AFLAPVMNLP EEHSRMVYFA ISDYAFNTAS LVYHKAGFLN FTITDDVIPP DSSIRQNTKS FRAFVPRIAR LYPNTNLELQ GAVISAPCLN FSPGNLSTAA QMEIEAFVLL PNSVKEPVFR LSVATNVSAM LTFNTSKITG FLEPGKIQVE LKESKVGRFN VELLEALLNY YLLNNFYPKV NDKLAEGFPL PLLRKIQLYD PILQIHKDFL FLGTNVRYLR V
• Sequence Length: 481

• Applicable Applications for LBP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Asn27~Val481 (Accession # Q2TBI0) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 84579853
• NCBI GeneID: 512242
• NCBI Accession #: NP_001033763.1
• NCBI GenBank Nucleotide #: NM_001038674.2
• UniProt Accession #: Q2TBI0
• Molecular Weight: 52.7kDa
• NCBI Official Full Name: lipopolysaccharide-binding protein
• NCBI Official Synonym Full Names: lipopolysaccharide binding protein<
• NCBI Official Symbol: LBP
• NCBI Protein Information: lipopolysaccharide-binding protein
• UniProt Protein Name: Lipopolysaccharide-binding protein
• Protein Family: Lipopolysaccharide-binding protein
• UniProt Gene Name: LBP
• UniProt Synonym Gene Names: LBP
• UniProt Entry Name: LBP_BOVIN

Uniprot Description

Function: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS

By similarity.

Subunit structure: When bound to LPS, interacts (via C-terminus) with soluble CD14

By similarity.

Subcellular location: Secreted

By similarity.

Sequence similarities: Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.