Immunohistochemistry (IHC)
(MBS9606886 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit FAM3B Polyclonal Antibody | anti-FAM3B antibody
FAM3B Antibody
Gene Names
FAM3B; 2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
FAM3B; Polyclonal Antibody; FAM3B Antibody; 2-21; C21orf11; C21orf76; Cytokine like protein 2 21; Cytokine-like protein 2-21; Fam3b; FAM3B_HUMAN; Family with sequence similarity 3 member B; ORF9; Pancreatic-derived factor; PANDER; PRED44; Protein FAM3B; anti-FAM3B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
FAM3B antibody detects endogenous levels of total FAM3B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
235
Applicable Applications for anti-FAM3B antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human FAM3B
Subcellular Location
Secreted. Localized in discrete vesicular and perinuclear structure.
Tissue Specificity
Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9606886 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9606886 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of PC-3 lysate using FAM3B antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of PC-3 lysate using FAM3B antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-FAM3B antibody
Description: FAM3B (family with sequence similarity 3, member B), also known as ORF9, C21orf11 or PANDER, is a 235 amino acid secreted protein that localizes to discrete vesicular and perinuclear structures. Expressed at high levels in pancreas and at lower levels in kidney, colon, testis, prostate and small intestine, FAM3B functions as an islet-specific cytokine that promotes apoptosis and may inhibit Insulin secretion from beta-cells, possibly playing a role in overall pancreatic activity. Human FAM3B shares 78% sequence identity with its mouse counterpart, suggesting a conserved role between species. Three isoforms of FAM3B, designated A, B and C, exist due to alternative splicing events. The gene encoding FAM3B maps to human chromosome 21, which houses approximately 300 genes and comprises nearly 1.5% of the human genome.
Function: Induces apoptosis of alpha and beta cells in a dose-and time-dependent manner.
Post-translational Modifications: 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. O-glycosylated.
Similarity: Belongs to the FAM3 family.
Function: Induces apoptosis of alpha and beta cells in a dose-and time-dependent manner.
Post-translational Modifications: 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. O-glycosylated.
Similarity: Belongs to the FAM3 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 26 kDa
Predicted: 26 kDa
Predicted: 26 kDa
NCBI Official Full Name
protein FAM3B isoform a
NCBI Official Synonym Full Names
family with sequence similarity 3 member B
NCBI Official Symbol
FAM3B
NCBI Official Synonym Symbols
2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
NCBI Protein Information
protein FAM3B
UniProt Protein Name
Protein FAM3B
Protein Family
UniProt Gene Name
FAM3B
UniProt Synonym Gene Names
C21orf11; C21orf76; PANDER
Uniprot Description
Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner.
Research Articles on FAM3B
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Product Notes
The FAM3B fam3b (Catalog #AAA9606886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAM3B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FAM3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FAM3B fam3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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