S100 Calcium Binding Protein A8 (S100A8) Recombinant Protein | S100A8 recombinant protein

Recombinant S100 Calcium Binding Protein A8 (S100A8)

Cat. Num. AAA2011482

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: S100 Calcium Binding Protein A8 (S100A8); Recombinant S100 Calcium Binding Protein A8 (S100A8); S100A8 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MLTDLECAIN SLIDVYHKYS LKKGNYHAVY RDDLKQLLET ECPKFMKKKD ADTWFKELDI NQDGGINFEE FLVLVIKVGL EAHEEIHKE
• Sequence Length: 89

• Applicable Applications for S100A8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Met1~Glu89 (Accession # P28782) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 165973998
• NCBI GeneID: 616818
• NCBI Accession #: NP_001107197.1
• NCBI GenBank Nucleotide #: NM_001113725.2
• UniProt Accession #: P28782; Q2NKR8
• Molecular Weight: 15.9kDa
• NCBI Official Full Name: protein S100-A8
• NCBI Official Synonym Full Names: S100 calcium binding protein A8<
• NCBI Official Symbol: S100A8
• NCBI Protein Information: protein S100-A8; P7; BEE11; calgranulin-A; S100 calcium-binding protein A8; neutrophil cytosolic 7 kDa protein
• UniProt Protein Name: Protein S100-A8
• Protein Family: Protein
• UniProt Gene Name: S100A8
• UniProt Synonym Gene Names: P7
• UniProt Entry Name: S10A8_BOVIN

Uniprot Description

Function: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants

By similarity.

Subunit structure: Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A9 known as calprotectin (S100A8/A9). S100A8 interacts with AGER, ATP2A2 and with the heterodimeric complex formed by TLR4 and LY96. Calprotectin (S100A8/9) interacts with CEACAM3 and tubulin filaments in a calcium-dependent manner. Heterotetrameric calprotectin (S100A8/A9) interacts with ANXA6 and associates with tubulin filaments in activated monocytes. S100A8 and calprotectin (S100A8/9) interact with NCF2/P67PHOX, RAC1 and RAC2. Calprotectin (S100A8/9) interacts with CYBA and CYBB

By similarity.

Subcellular location: Secreted

By similarity. Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity. Cell membrane; Peripheral membrane protein

By similarity. Note: Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway

By similarity.

Tissue specificity: Found essentially in phagocytic cells.

Miscellaneous: Binds two calcium ions per molecule with an affinity similar to that of the S100 proteins

By similarity.

Sequence similarities: Belongs to the S-100 family.Contains 2 EF-hand domains.