LAIR1 recombinant protein

Recombinant Mouse LAIR1 Protein (Fc tag)

Cat. Num. AAA2546687

• Gene Names: Lair1; Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: LAIR1; Recombinant Mouse LAIR1 Protein (Fc tag); LAIR1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 263

• Application Notes: The recombinant mouse LAIR1 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 353 amino acids and has a predicted molecular mass of 39.6 kDa. The apparent molecular mass of the protein is approximately 49-53 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gln 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LAIR1 recombinant protein

Background: Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells. 

Description: A DNA sequence encoding the mouse LAIR1 (Q8BG84-6) (Gln22-Ser133) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 164698422
• NCBI GeneID: 52855
• NCBI Accession #: NP_001106945.1
• NCBI GenBank Nucleotide #: NM_001113474.1
• UniProt Accession #: Q8BG84; Q80UN5; Q8BHB6; Q8BRR6; Q8C6N8; Q8CEP5
• Molecular Weight: 25,128 Da
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform a
• NCBI Official Synonym Full Names: leukocyte-associated Ig-like receptor 1
• NCBI Official Symbol: Lair1
• NCBI Official Synonym Symbols: Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: Lair1
• UniProt Synonym Gene Names: LAIR-1; mLAIR1

Uniprot Description

LAIR-1: an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region called the leukocyte receptor cluster, which contains at least 25 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 7 A1|7 2.31 cM

Cellular Component: plasma membrane