Rabbit anti-Rat LAIR1 Polyclonal Antibody | anti-LAIR1 antibody

Anti-LAIR1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101646

• Reactivity: Rat
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: LAIR1; Polyclonal Antibody; Anti-LAIR1 Antibody; Rabbit Polyclonal; LAIR1/LAIR-1/CD305 Antibody; Rabbit PAb; Antigen Affinity Purified; leukocyte-associated immunoglobulin-like receptor 1; anti-LAIR1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat LAIR1/LAIR-1/CD305
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LAIR1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Rat LAIR1/LAIR-1/CD305
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Rat LAIR1/LAIR-1/CD305, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-LAIR1 rabbit polyclonal antibody at 1:500 dilution.Lane A: mouse liver tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa)

Related Product Information for anti-LAIR1 antibody

Leukocyte-associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and can spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.

Product Categories/Family for anti-LAIR1 antibody

ITIM/ITAM Immunoreceptors and Related Molecules

References

Meyaard L, et al. (1999) Leukocyte-associated Ig-like receptor-1 functions as an inhibitory receptor on cytotoxic T cells. J Immunol. 162 (10): 5800-4.Meyaard L, et al. (2001) The epithelial cellular adhesion molecule (Ep-CAM) is a ligand for the leukocyte-associated immunoglobulin-like receptor (LAIR). J Exp Med. 194 (1): 107-12.Xu M, et al. (2000) Identification and characterization of leukocyte-associated Ig-like receptor-1 as a major anchor protein of tyrosine phosphatase SHP-1 in hematopoietic cells. J Biol Chem. 275 (23): 17440-6.

NCBI and Uniprot Product Information

• NCBI GI #: 153945730
• NCBI GeneID: 574531
• NCBI Accession #: NP_001025099.1
• NCBI GenBank Nucleotide #: NM_001029928.1
• UniProt Accession #: P0C1X9
• Molecular Weight: 29,724 Da
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Synonym Full Names: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Symbol: Lair1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1; LAIR-1; rLAIR1; leukocyte-associated Ig-like receptor 1; leukocyte-associated Ig-like receptor-1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: Lair1
• UniProt Synonym Gene Names: LAIR-1; rLAIR1
• UniProt Entry Name: LAIR1_RAT

Uniprot Description

Function: Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells