Rabbit anti-Mouse, Rat Ephrin-B2 Polyclonal Antibody | anti-B2 antibody

Ephrin-B2 Polyclonal Antibody

Cat. Num. AAA3009078

• Gene Names: EFNB2; HTKL; EPLG5; Htk-L; LERK5
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ephrin-B2; Polyclonal Antibody; Ephrin-B2 Polyclonal Antibody; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L; EFNB2; EPLG5; HTKL; LERK5; anti-B2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ephrin-B2 polyclonal antibody detects endogenous levels of Ephrin-B2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 333

• Applicable Applications for anti-B2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human Ephrin-B2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of Ephrin-B2 polyclonal antibody)

Related Product Information for anti-B2 antibody

Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.

NCBI and Uniprot Product Information

• NCBI GI #: 4758250
• NCBI GeneID: 1948
• NCBI Accession #: NP_004084.1
• NCBI GenBank Nucleotide #: NP_004084.1
• UniProt Accession #: P52799
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: ephrin-B2 isoform 1
• NCBI Official Synonym Full Names: ephrin B2
• NCBI Official Symbol: EFNB2
• NCBI Official Synonym Symbols: HTKL; EPLG5; Htk-L; LERK5
• NCBI Protein Information: ephrin-B2
• UniProt Protein Name: Ephrin-B2
• Protein Family: Ephrin
• UniProt Gene Name: EFNB2
• UniProt Synonym Gene Names: EPLG5; HTKL; LERK5; LERK-5; HTK-L
• UniProt Entry Name: EFNB2_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]