Lair1 recombinant protein

Lair1, 22-144aa, Mouse, His-tag, Baculovirus

Cat. Num. AAA206453

• Gene Names: Lair1; Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Lair1; 22-144aa; Mouse; His-tag; Baculovirus; Leukocyte-associated immunoglobulin-like receptor 1 isoform a; 5133400O11Rik; BB115266; D7Bwg0421e; Lair-1; Lair1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPQEGSLPD ITIFPNSSLM ISQGTFVTVV CSYSDKHDLY NMVRLEKDGS TFMEKSTEPY KTEDEFEIGP VNETITGHYS CIYSKGITWS ERSKTLELKV IKENVIQTPA PGPTSDTSWL KTYSIYHHHH HH
• Sequence Length: 263

• Applicable Applications for Lair1 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Lair1 recombinant protein

Lair1, as known as leukocyte-associated immunoglobulin-like receptor 1 isoform a, is an inhibitory receptor of the lg superfamily that is related to inhibitory members of KIR and ILT/CD85 families. This protein is well expressed NK cells, T cells, B cells, monocytes, macrophages, immature neutrophils, dendritic cells and most thymocytes. Also, it functions as an inhibitory receptor not only on NK cells, but on human T cells. Recombinant mouse Lair1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Lair1 recombinant protein

Immunology

References

Tang X., et al. (2012) J. Immunol. 188:548-558.; Jiang L., et al. (209) Eur. J. Immunol. 39:1167-1175.

NCBI and Uniprot Product Information

• NCBI GI #: 164698422
• NCBI GeneID: 52855
• NCBI Accession #: NP_001106945
• NCBI GenBank Nucleotide #: NM_001113474.1
• UniProt Accession #: Q8BG84; Q80UN5; Q8BHB6; Q8BRR6; Q8C6N8; Q8CEP5
• Molecular Weight: 15.0kDa (132aa) 18-28kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform a
• NCBI Official Synonym Full Names: leukocyte-associated Ig-like receptor 1
• NCBI Official Symbol: Lair1
• NCBI Official Synonym Symbols: Lair-1; BB115266; D7Bwg0421e; 5133400O11Rik
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: Lair1
• UniProt Synonym Gene Names: LAIR-1; mLAIR1

Uniprot Description

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells ().